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Intracellular Raman spectroscopy sensing based on resonant photonic structures

Research Area: Photonic integrated circuits for the visible/near IR , Silicon photonics for lab-on-chip spectroscopy

Main Researcher: Pieter Wuytens

The detection of subcellular molecules is crucial for both understanding cellular processes and developing new drugs. The use of fluorescent labels has been of paramount importance for visualizing specifically targeted molecule, but is limited by the labels itself. Recently, labelfree methods for intracellular sensing have gained large interested because of their strong multiplexing capacities and fingerprint specificity. The latter argument is particularly valid for Raman Spectroscopy. However, Raman spectroscopy produces extremely low signals. Current methods using colloidal gold nanoparticles for enhancing the Raman signal suffer from a poor reproducibility and a highly nonuniform enhancement.
In this project we investigate a new way for enhancing the intracellular Raman signal using cavity resonances in silicon nitride photonic chips. This interdisciplinary PhD is a joint project between the Department of Molecular Biotechnology (prof. AG Skirtach) and the Photonics Research Group, combining expertise on cell biology with photonic integration under the framework of NB Photonics.

Other people involved:

Related Research Projects


    International Journals

  1. Z. Liu, J. Zhang, G. Roelkens, N. Le Thomas, R. Baets, Bottom-reflector enhanced grating couplers micro-transfer printed on silicon nitride photonic integrated circuits, accepted for publication in Journal of Lightwave Technology,  (to be published).
  2. E. Lengert, M. Saveleva, A. Vsevolod, P.C. Wuytens, R. Kamyshinsky, A. Vasiliev, D. Gorin, G. Sukhorukov, A. G. Skirtach, B. Parakhonskiy, Silver alginate hydrogel micro- and nano- containers for theranostics: synthesis, encapsulation, remote release and detection, ACS Applied Materials & Interfaces, 2017(9), p.21949-21958 doi:10.1021/acsami.7b08147 (2017)  Download this Publication (6.8MB).
  3. P.C. Wuytens, A. Subramanian, W. H. De Vos, A. G Skirtach, R. Baets, Gold nanodome-patterned microchips for intracellular surface-enhanced Raman spectroscopy, Analyst, 140(24), p.8080-8087 doi:10.1039/c5an01782c (2015)  Download this Publication (5.7MB).
  4. A. Subramanian, E.M.P. Ryckeboer, A. Dhakal, F. Peyskens, A. Malik, B. Kuyken, H. Zhao, S. Pathak, A. Ruocco, A. De Groote, P.C. Wuytens, D. Martens, F. Leo, W. Xie, U.D. Dave, M. Muneeb, Pol Van Dorpe, J. Van Campenhout, W. Bogaerts, P. Bienstman, N. Le Thomas, D. Van Thourhout, Zeger Hens, G. Roelkens, R. Baets, Silicon and silicon nitride photonic circuits for spectroscopic sensing on-a-chip , Photonics Research (invited), 5(3), p.B47 doi:10.1364/PRJ.3.000B47 (2015)  Download this Publication (1.5MB).
  5. P.C. Wuytens, B. Parakhonskiy, A. Yashchenok, M. Winterhalter, A. Skirtach, Pharmacological aspects of release from microcapsules—from polymeric multilayers to lipid membranes, Current Opinion in Pharmacology, 18, p.129-140 doi:10.1016/j.coph.2014.09.016 (2014)  Download this Publication (1.6MB).
      International Conferences

    1. Z. Wang, M. Pantouvaki, G. Morthier, C. Merckling, J. Van Campenhout, D. Van Thourhout, G. Roelkens, Heterogeneous Integration of InP Devices on Silicon, the 28th International Conference on Indium Phosphide and Related Materials (IPRM) (invited), Japan, p.paper ThD1-1 (2016)  Download this Publication (379KB).
    2. P.C. Wuytens, W.H. Vos, A. Skirtach, R. Baets, Nanodome coins for intracellular surface-enhanced Raman spectroscopy, 3rd International Conference on BioPhotonics, Italy, p.paper P1.30 doi:10.1109/biophotonics.2015.7304044 (2015)  Download this Publication (552KB).
    3. P.C. Wuytens, A. M. Yashchenok, A. Subramanian, A. G. Skirtach, R. Baets, Micron - sized dielectric chips with gold nanostructures for intracellular label - free SERS, 19 th Annual Symposium of the IEEE Photonics Society Benelux Chapter, (2014)  Download this Publication (1.9MB).
    4. P.C. Wuytens, A. M. Yashchenok, A. Subramanian, R. Baets, A.G. Skirtach, Label-free monitoring of microcapsule-enabled intracellular release using goldnanoparticle coated microchips, Surface Enhanced Spectroscopies 2014, Germany, p.158 (2014)  Download this Publication (1.4MB).
    5. P.CU Wuytens, A. M. Yashchenok, A. Subramanian, A. G. Skirtach, R. Baets, Gold Nanoparticle Coated Silicon Nitride chips for Intracellular Surface-Enhanced Raman Spectroscopy, CLEO, STh4H.7, United States, doi:10.1364/cleo_si.2014.sth4h.7 (2014)  Download this Publication (217KB).

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